Pengaruh Intermittent Fasting terhadap Penurunan Ekspresi mRNA Gen Tumor Necrosis Factor-Alpha pada Mencit (Mus Musculus)

Muhammad Basir; Mochammad Hatta; Suryani As’ad; Ilhamjaya Patellongi

doi:10.30595/pshms.v7i.1454

Authors

Muhammad Basir Universitas Megarezky Makassar, Indonesia
Mochammad Hatta Universitas Hasanuddin Makassar, Indonesia
Suryani As’ad Universitas Hasanuddin Makassar, Indonesia
Ilhamjaya Patellongi Universitas Hasanuddin Makassar, Indonesia

DOI:

https://doi.org/10.30595/pshms.v7i.1454

Keywords:

Intermittent Fasting, TNF-?

Abstract

Saat ini perkembangan berbagai penyakit degeneratif sangat pesat. Pesatnya perkembangan penyakit tersebut telah mendorong masyarakat luas untuk memahami dampak yang ditimbulkannya. Intermittent fasting bisa membantu mencegah munculnya kanker (salah satu penyakit degeneratif) karena selama puasa sel-sel tubuh berada dalam mode protektif sehingga menjaga tubuh dari serangan penyakit. Intermittent fasting mengurangi status inflamasi tubuh dengan menghambat ekspresi sitokin proinflamasi terutama Tumor Necrosis Factor Alpha (TNF-?). Penelitian ini bertujuan menguji pengaruh intermittent fasting terhadap ekspresi mRNA gen TNF-?. Penelitian ini adalah experiment dengan rancangan Pretest-Posttest with Control Group untuk menguji adanya pengaruh intermittent fasting terhadap ekspresi mRNA Gen TNF-?. Subyek penelitian ini adalah 10 ekor mencit yang dibagi 2 kelompok yaitu kelompok perlakuan (intermittent fasting) dan kelompok kontrol. Penelitian dilaksanakan di Laboratorium Imunologi dan Biologi Molekuler Bagian Mikrobiologi Fakultas Kedokteran Universitas Hasanuddin Makassar sebagai tempat pemeliharaan dan perawatan hewan coba serta pemeriksaan ekspresi mRNA Gen TNF-?. Penelitian dilaksanakan selama 30 hari. Hasil penelitian memperlihatkan adanya penurunan rerata nilai ekspresi mRNA gen TNF-? subyek sebelum dan setelah intermittent fasting dengan nilai ?=0,013 (?<0,05) menunjukkan bahwa ada pengaruh yang bermakna antara intermittent fasting terhadap penurunan ekspresi mRNA Gen TNF-?. Kesimpulannya terjadi penurunan ekspresi mRNA gen TNF-? setelah intermittent fasting.

References

Dinkes RI. (2018). Riset Kesehatan Dasar 2018.

A. Abbas, A. Lichtman, S.Pillai. (2015). Cellular and molecular immunology. International Edition, 8th. ISBN: 9780323315906

Hasanian-Mehr, M., Ebrahimi, S., Rahmani, F., Avan, A., Nematy, M., Mostafa Parizadeh, S., & Mohammad Reza Parizadeh, S. (2016). Effects of Ramadan Fasting on the Regulation of Inflammation. J Fasting Health, 4(1), 32–37. Retrieved from http://jfh.mums.ac.ir/article_6624_d49a33cb785a5e5f42c5392fa8f363b5.pdf

Aliasghari, F., Izadi, A., Gargari, B. P., & Ebrahimi, S. (2017). The Effects of Ramadan Fasting on Body Composition, Blood Pressure, Glucose Metabolism, and Markers of Inflammation in NAFLD Patients: An Observational Trial. Journal of the American College of Nutrition. https://doi.org/10.1080/07315724.2017.1339644

St. Pierre, B., & Berardi, J. (2014). Intermittent Fasting: A Primer. IDEA Fitness Journal.

Ünalacak, M., Kara, ?. H., Baltaci, D., Erdem, Ö., & Bucaktepe, P. G. E. (2011). Effects of Ramadan Fasting on Biochemical and Hematological Parameters and Cytokines in Healthy and Obese Individuals. Metabolic Syndrome and Related Disorders. https://doi.org/10.1089/met.2010.0084

Effendi, Z. (2013). Peranan Leukosit Sebagai Anti Inflamasi Alergik Dalam Tubuh. Repository USU, 1–8.

Akrami Mohajeri, F., Ahmadi, Z., Hassanshahi, G., Akrami Mohajeri, E., Ravari, A., & Razi Ghalebi, S. (2013). Dose Ramadan fasting affects inflammatory responses: Evidences for modulatory roles of this unique nutritional status via chemokine network. Iranian Journal of Basic Medical Sciences.

Jahromi, et al. (2014). Islamic fasting and multiple sclerosis. BMC Neurology. https://doi.org/10.1186/1471-2377-14-56

Nematy, et al. (2012). Effects of Ramadan fasting on cardiovascular risk factors: A prospective observational study. Nutrition Journal. https://doi.org/10.1186/1475-2891-11-69

Lahdimawan, Ardik Handono, Kusworini Rasjad Indra, M Prawiro, Sumarno Reto. (2013). Effect of Ramadan Fasting On Classically Activated, Oxidative Stress and Inflammation of Macrophage. IOSR Journal Of Pharmacy Www.Iosrphr.Org

Wan, Ruiqian Ahmet, Ismayil Brown, Martin Cheng, Aiwu Kamimura, Naomi Talan, Mark Mattson, Mark P. (2010). Cardioprotective effect of intermittent fasting is associated with an elevation of adiponectin levels in rats. The Journal of nutritional biochemistry. https://doi.org/10.1016/j.jnutbio.2009.01.020

Castello, L., Froio, T., Maina, M., Cavallini, G., Biasi, F., Leonarduzzi, G., … Chiarpotto, E. (2010). Alternate-day fasting protects the rat heart against age-induced inflammation and fibrosis by inhibiting oxidative damage and NF-kB activation. Free Radical Biology and Medicine. https://doi.org/10.1016/j.freeradbiomed.2009.10.003

Faris, M. A.-I. E., Kacimi, S., Al-Kurd, R. a, Fararjeh, M. a, Bustanji, Y. K., Mohammad, M. K., & Salem, M. L. (2012). Intermittent fasting during Ramadan attenuates proinflammatory cytokines and immune cells in healthy subjects. Nutrition Research (New York, N.Y.). https://doi.org/10.1016/j.nutres.2012.06.021