Synthesis of Gold Nanoparticles Using Pegagan Leaf Extract as Reductor and Tyrosinase Enzyme Inhibitor Activity Test
DOI:
https://doi.org/10.30595/pshms.v9i1.2215Keywords:
Gold nanoparticle, pegagan extract, characterization, inhibitor activity testingAbstract
Introduction: Nanoparticles ranging from 1-100 nm in diameter can be synthesized using green synthesis methods, which utilize environmentally friendly, non-toxic, economical, and easily manageable resources to promote sustainable nanotechnology development. Pegagan contains saponins, triterpenoids, flavonoids, and tannins that serve as antioxidants for the skin, offering anti-inflammatory and anti-aging benefits. This study aims to synthesize and characterize gold nanoparticles using pegagan leaf extract, and evaluate its potential as an inhibitor of the tyrosinase enzyme involved in melanin formation in human skin.
Methods: Synthesis was conducted by mixing HAuCl4 at a concentration of 2 mM with liquid extract of pegagan leaves. The synthesized products were characterized using UV-Vis spectrophotometer, spectrofluorometer, and Particle Size Analyzer to observe the characteristics of the formed nanoparticles. Additionally, the inhibition activity of the tyrosinase enzyme was tested using a microplate reader at a maximum wavelength of 490 nm.
Results: During the synthesis of gold nanoparticles, there was a color change in the solution from yellow to purple. Measurements using UV-Vis spectrophotometer showed a spectrum with a peak wavelength at 533 nm, but no fluorescence was observed. Gold nanoparticles measured using PSA ranged in size from 24.36 to 91.28 nm. The inhibition activity test of the CA-AuNPs solution yielded an IC50 of -2,286.202 µg/ml, while the positive control solution using kojic acid showed an IC50 of 31.615 µg/ml.
Conclusion: Gold nanoparticles can be synthesized using pegagan extract as a reducer, but they do not exhibit potential as inhibitors of the tyrosinase enzyme.
References
[1] Abdassah, M. (2017). Nanopartikel Dengan Gelasi ionik. Jurnal Farmaka, 15(1), 45–52.
[2] Aprilliani, A. (2018). Uji Inhibisi Aktivitas Enzim Tirosinase Beberapa Jenis Tumbuhan Anggota Suku Zingiberaceae. Jurnal Ilmiah Farmasi, 14(1), 46–57. https://doi.org/10.20885/jif.vol14.iss1.art05
[3] Batubara, I., Darusman, L. K., Mitsunaga, T., Rahminiwati, M., & Djauhari, E. (2010). Potency of Indonesian Medicinal Plants as Tyrosinase Inhibitor and Antioxidant Agent. In Journal of Biological Sciences (Vol. 10, Issue 2, pp. 138–144). https://doi.org/10.3923/jbs.2010.138.144
[4] Bharadwaj, K. K., Rabha, B., Pati, S., Sarkar, T., Choudhury, B. K., Barman, A., Bhattacharjya, D., Srivastava, A., Baishya, D., Edinur, H. A., Kari, Z. A., & Noor, N. H. M. (2021). Green Synthesis of Gold Nanoparticles Using Plant Extracts as Beneficial Prospect for Cancer Theranostics. Molecules, 26(21). https://doi.org/10.3390/molecules26216389
[5] Buzea, C., Pacheco, I. I., & Robbie, K. (2007). Nanomaterials and Nanoparticles: Sources and Toxicity. Biointerphases, 2(4), MR17–MR71. https://doi.org/10.1116/1.2815690
[6] Chang, T. S. (2009). An Updated Review of Tyrosinase Inhibitors. International Journal of Molecular Sciences, 10(6), 2440–2475. https://doi.org/10.3390/ijms10062440
[7] Chang, T. S. (2012). Natural Melanogenesis Inhibitors Acting Through The Down-Regulation of Tyrosinase Activity. Materials, 5(9), 1661–1685. https://doi.org/10.3390/ma5091661
[8] Dachriyanus. (2004). Analisis Struktur Senyawa Organik Secara Spektroskopi.
[9] Das, J., & Velusamy, P. (2014). Catalytic Reduction of Methylene Blue Using Biogenic Gold Nanoparticles From Sesbania grandiflora L. Journal of the Taiwan Institute of Chemical Engineers, 45(5), 2280–2285. https://doi.org/10.1016/j.jtice.2014.04.005
[10] Das, R. K., Borthakur, B. B., & Bora, U. (2010). Green Synthesis of Gold Nanoparticles Using Ethanolic Leaf Extract of Centella asiatica. Materials Letters, 64(13), 1445–1447. https://doi.org/10.1016/j.matlet.2010.03.051
[11] DEPKES. (2017). Farmakope Herbal Indonesia Edisi II 2017. Kementerian Kesehatan Republik Indonesia. https://doi.org/10.2307/jj.2430657.12
[12] Fazrin, E. I., Naviardianti, A. I., Wyantuti, S., Gaffar, S., & Hartati, Y. W. (2020). Review: Sintesis Dan Karakterisasi Nanopartikel Emas (AuNP) Serta Konjugasi AuNP Dengan DNA Dalam Aplikasi Biosensor Elektrokimia. PENDIPA Journal of Science Education, 4(2), 21–39. https://doi.org/10.33369/pendipa.4.2.21-39
[13] Fernenda, L., Ramadhani, A. P., & Syukri, Y. (2023). Aktivitas Pegagan (Centella asiatica) Pada Dermatologi. Jurnal Sains Farmasi & Klinis, 9(3), 237. https://doi.org/10.25077/jsfk.9.3.237-244.2022
[14] Frens, G., kolloid Z. (1973). Controlled Nucleation for the Regulation of the Particle Size in Monodisperse Gold Suspensions. In Nat. Phys. Sci. (Vol. 241).
[15] Gazali, M., Zamani, N. P., & Batubara, I. (2014). Potensi Limbah Kulit Buah Nyirih Xylocarpus granatum Sebagai Inhibitor Tirosinase. June 2017. https://doi.org/10.13170/depik.3.3.2153
[16] Hebbar, S., Dubey, A., Ravi, G. S., Kumar, H., & Saha, S. (2019). RP-HPLC Method Development and Validation of Asiatic acid Isolated From The Plant Centella asiatica. International Journal of Applied Pharmaceutics, 11(3), 72–78. https://doi.org/10.22159/ijap.2019v11i3.31525
[17] Jahan, R., Hossain, S., Seraj, S., Nasrin, D., Khatun, Z., Das, P. R., Md. Islam, T., Ahmed, I., & Rahmatullah, M. (2012). Centella asiatica (L.) Urb.: Ethnomedicinal Uses and Their Scientific Validations. American-Eurasian Journal of Sustainable Agriculture, 6(4), 261–270.
[18] Jhansi, D., & Kola, M. (2019). The Antioxidant Potential of Centella asiatica?: A review. 7(2), 18–20.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Proceedings Series on Health & Medical Sciences
This work is licensed under a Creative Commons Attribution 4.0 International License.
