Produksi Coconut Vinegar dari Limbah Air Kelapa dengan Model Fermentasi Cair Termodifikasi Sel Yeast Terimobilisasi Kalsium Alginat
DOI:
https://doi.org/10.30595/pspfs.v6i.853Kata Kunci:
Coconut vinegar, Fermentasi, Media Cair SImultan, Yeast Terimobisasi AlginatAbstrak
Coconut (Cocos Nucifera L) is one of the Indonesia’s plantations comodities. Unfortunately, the abundance of raw materials has not had a significant impact on the export market for coconut products, especially functional food products. Based on that issue, research with the aim to produce diversified product of coconut was conducted. The product developed in this research was coconut vinegar produced by fermentation process of coconut waste water via liquid fermentation pathway. To increase efficiency and reduce production costs, the fermentation process was carried out simultaneously. In order to increase the yeast resistance to acetic acid produced by acetic acid bacteria (BAA), the process modification was conducted by using yeast immobilized formed by the calcium alginate matrix. The research results show that the optimum BAA inoculation time was 24 hours after the bead yeast inoculation. The maximum bead yeast mass obtained in this study was 25% m/v. Fermentation that was carried out with the maximum time and bead mass was able to produce coconut vinegar with an acetic acid content of 5.1 g/L, an alcohol conversion of 22.73%.
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Referensi
Adler, P., Frey, L. J., Berger, A., Bolten, C. J., Hansen, C. E., & Wittmann, C. (2014). The key to acetate: Metabolic fluxes of acetic acid bacteria under cocoa pulp fermentation-simulating conditions. Applied and Environmental Microbiology, 80(15), 4702–4716. https://doi.org/10.1128/AEM.01048-14
Awaltanova,Ella., Syaiful, BAhri., Chairul., 2015, “Fermentasi Nira Nipah Menjadi Bioetanol Menggunakan Teknik Immobilisasi Sel Saccharomyces Cerevisiae”, JOM FTEKNIK Volume 2 No. 2.
Jiménez-Hornero, J. E., Santos-Dueñas, I. M., & García-García, I. (2009). Optimization of biotechnological processes. The acetic acid fermentation. Part I: The proposed model. Biochemical Engineering Journal, 45(1), 1–6. https://doi.org/10.1016/j.bej.2009.01.009
Kanchanarach, W., Theeragool, G., Inoue, T., Yakushi, T., Adachi, O., & Matsushita, K. (2010). Acetic acid fermentation of acetobacter pasteurianus: Relationship between acetic acid resistance and pellicle polysaccharide formation. Bioscience, Biotechnology and Biochemistry, 74(8), 1591–1597. https://doi.org/10.1271/bbb.100183
Krusong, W., Yaiyen, S., & Pornpukdeewatana, S. (2015). Impact of high initial concentrations of acetic acid and ethanol on acetification rate in an internal Venturi injector bioreactor. Journal of Applied Microbiology, 118(3), 629–640. https://doi.org/10.1111/jam.12715
Krusong, W., & Vichitraka, A. (2010). An investigation of simultaneous pineapple vinegar fermentation interaction between acetic acid bacteria and yeast Warawut. Asian Journal of Food and Agro-Industry, 3(01), 192–203.
Poreda, A., Tuszy?ski, T., Zdaniewicz, M., Sroka, P., & Jakubowski, M. (2013). Support materials for yeast immobilization affect the concentration of metal ions in the fermentation medium. Journal of the Institute of Brewing, 119(3), 164–171. https://doi.org/10.1002/jib.77
Roni, K. A., Kartika, D., Apriyadi, H., & Herawati, N. (2019). The effect of type and concentration yeast with fermentation time and liquifaction variations on the bioethanol concentration resulted by sorgum seeds with hydrolysis and fermentation processes. Journal of Computational and Theoretical Nanoscience, 16(12), 5228–5232. https://doi.org/10.1166/jctn.2019.8591
Saeki, A., Taniguchi, M., Matsushita, K., Toyama, H., Theeragool, G., Lotong, N., & Adachi, O. (1997). Microbiological aspects of acetate oxidation by acetic acid bacteria, unfavorable phenomena in vinegar fermentation. Bioscience, Biotechnology and Biochemistry, 61(2), 317–323. https://doi.org/10.1271/bbb.61.317
Siregar, J. S., Ahmad, A., & Amraini, S. Z. (2019). Effect of Time Fermentation and Saccharomyces Cerevisiae Concentration for Bioethanol Production from Empty Fruit Bunch. Journal of Physics: Conference Series, 1351(1). https://doi.org/10.1088/1742-6596/1351/1/012104
Sousa-Dias, M. L., Paula, V. B., Dias, L. G., & Estevinho, L. M. (2021). Mead production using immobilized cells of saccharomyces cerevisiae: Reuse of sodium alginate beads. Processes, 9(4). https://doi.org/10.3390/pr9040724
Sroka, P., Satora, P., Tarko, T., & Duda-Chodak, A. (2017). The influence of yeast immobilization on selected parameters of young meads. Journal of the Institute of Brewing, 123(2), 289–295. https://doi.org/10.1002/jib.409
Tanamool, V., Chantarangsee, M., & Soemphol, W. (2020). Simultaneous vinegar fermentation from a pineapple by-product using the co-inoculation of yeast and thermotolerant acetic acid bacteria and their physiochemical properties. 3 Biotech, 10(3), 1–11. https://doi.org/10.1007/s13205-020-2119-4
Xia, K., Zang, N., Zhang, J., Zhang, H., Li, Y., Liu, Y., Feng, W., & Liang, X. (2016). New Insights Into The Mechanisms Of Acetic Acid Resistance In Acetobacter Pasteurianus Using Itraq-Dependent Quantitative Proteomic Analysis. International Journal of Food Microbiology, 238, 241–251. https://doi.org/10.1016/j.ijfoodmicro.2016.09.016
Zhao, J., Wang, Z., Wang, M., He, Q., & Zhang, H. (2008). The inhibition of Saccharomyces cerevisiae cells by acetic acid quantified by electrochemistry and fluorescence. Bioelectrochemistry, 72(2), 117–121. https://doi.org/10.1016/j.bioelechem.2007.11.015
Zulaikhah, S. T. (2019). Health Benefits Of Tender Coconut Water (Tcw) Siti Thomas Zulaikhah Department of Public Health, Faculty of Medicine, UNISSULA, Semarang, Central Java, Indonesia. International Journal of Pharmaceutical Sciences and Research, 10(2), 474–480.
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