The Effectiveness of Providing Pineapple Core Gel (Ananas Comosus (L.) Merr) on Burns Healing Degree II in White Rats (Rattus Novergicus)

Atiek Murharyati; Joko Santoso; Erinda Nur Pratiwi; Sahuri Teguh Kurniawan; S. Dwi Sulisetyowati

Authors

Atiek Murharyati Kusuma Husada University Surakarta
Joko Santoso Kusuma Husada University Surakarta
Erinda Nur Pratiwi Kusuma Husada University Surakarta
Sahuri Teguh Kurniawan Kusuma Husada University Surakarta
S. Dwi Sulisetyowati Kusuma Husada University Surakarta

Keywords:

burn healing, gel, pineapple core

Abstract

Pineapple core can be an alternative in accelerating wound healing. Pineapple contains a proteolytic enzyme of bromelain, predominantly located in the pineapple core (Ananas comosus (L.) Merr). It has anti-inflammatory properties needed in the wound healing process, accelerates the inflammatory phase of the wound, and hopefully will accelerate it during the proliferative phase. The study aimed to analyze the effectiveness of pineapple core gel in recovering second-degree burns. This research formulated pineapple core as a moisturizer in gel preparations. The research methods employed an experimental study of pre-posttest with a control group design. They utilized 25 laboratory white mice (Rattus Novergicus) divided into five groups (each group 5 mice). The sample consisted of 5 treatment groups, namely group I (10% concentration), group II (20% concentration), group III (30% concentration), group IV (bioplacenton/positive control) and group V (negative control). The research obtained gel stability test of group I (viscosity 2.55 poise, pH 5.25, spreadability 5, comogen and gel dosage form), group II (viscosity 2.75 poise, pH 5.4, spreadability 5, comogen and shape gel preparation), group III (viscosity 2.8 poise, pH 5.2, spreadability 5, comogen and gel dosage form). In conclusion, group III (30% concentration) had more significant burn healing and a more stable stability test against storage than other groups.

References

Agrawal, P., Nikhade, P., Patel, A., Mankar, N., & Sedani, S. (2022). Bromelain: A Potent Phytomedicine. Cureus, 14(8), 1–7. https://doi.org/10.7759/cureus.27876

Fissore, A., Marengo, M., Santoro, V., Grillo, G., Oliaro-Bosso, S., Cravotto, G., Dal Piaz, F., & Adinolfi, S. (2023). Extraction and Characterization of Bromelain from Pineapple Core: A Strategy for Pineapple Waste Valorization. Processes, 11(7), 1–10. https://doi.org/10.3390/pr11072064

Fitri, R. M., Lubis, M. S., Dalimunthe, G. I., & Yuniarti, R. (2023). Skrining fitokimia, formulasi dan uji mutu fisik nanoserum ekstrak bonggol nanas (Ananas comosus (L.) Merr). Journal of Pharmaceutical and Sciences, 6(3), 1346–1355. https://doi.org/10.36490/journal-jps.com.v6i3.207

Ilyas, N. M. (2020). Isolasi dan Karakterisasi Enzim Bromelain dari Bonggol dan Daging Buah Nanas (Ananas comosus). Chemica: Jurnal Ilmiah Kimia Dan Pendidikan Kimia, 21(2), 133. https://doi.org/10.35580/chemica.v21i2.17983

Ji, S., Xiao, S., Xia, Z., & Association, C. B. (2023). Consensus on the treatment of second-degree burn wounds (2024 edition) Tissue Repair of Burns and Trauma Committee, Cross-Straits Medicine Exchange Association of China. In Burns & Trauma (Vol. 12, pp. 1–36). https://doi.org/https://doi.org/10.1093/burnst/tkad061

Laurens Acevedo, M., Usua, G. M., & Barret, J. P. (2025). Pain Management in Burned Patients Treated with Bromelain-Based Enzymatic Debridement. Journal of Clinical Medicine, 14(5), 1–10. https://doi.org/10.3390/jcm14051571

Minarni, M. (2019). Pengaruh Berkumur Dengan Maserasi Ekstrak Bonggol Nanas Terhadap Ph Saliva Rongga Mulut. Jurnal Kesehatan Gigi, 6(1), 63. https://doi.org/10.31983/jkg.v6i1.4435

Mosayan, G. A., Hukma S, S., & Walidah P H, H. (2022). Pinaplast: Plester Luka dari Ekstrak Bonggol Nanas (Ananas Comosus (L.) Merr.) sebagai Pengobatan Alami Luka Sayat. Jurnal Edukasi Dan Sains Biologi, 4(1), 26–33. https://doi.org/10.37301/esabi.v4i1.24

Mossfika, E., Abeiasa, M. S., & Putra, D. J. (2024). Perbandingan Aktivitas Antibakteri Ekstrak Bonggol Nanas (Ananas comosus L.) dan Getah Pepaya (Carica papaya) Terhadap Bakteri Stapylococcus Aureus. Jurnal Medisains Kesehatan, 5(1), 1–8. https://doi.org/10.59963/jmk.v5i1.294

Mulyani, L. N., Larasti, V., Handayani, D., & Herlina, H. (2024). Isolasi Dan Karakterisasi Bromelain Bonggol Nanas Dari Limbah Industri (Ananas comosus L. Merr.). Jurnal Farmasi Galenika, 11(1), 31–49. https://doi.org/10.70410/jfg.v11i1.328

Ng, C., Hamzah, M. S. A., & Nayan, N. H. M. (2023). Potential Role of Bromelain in Wound Healing Application: A Review. International Journal of Integrated Engineering, 15(4), 1–18. https://doi.org/10.30880/ijie.2023.15.04.001

Rusmini, H., Djunishap, A., Naufal, M. N. N., & Hanif, M. F. (2019). Pengaruh Gel Kulit Nanas Madu Terhadap Penyembuhan Luka Terbakar Derajat Dua Pada Tikus Putih (Rattus Novergicus). Sriwijaya Journal of Medicine, 2(3), 144–148. https://doi.org/10.32539/sjm.v2i3.73

Shah, K. C., & Srivastava, S. N. (2024). Bromelain based enzymatic debridement in burn patients, its outcome, advantages and disadvantages. International Surgery Journal, 11(11), 1837–1841. https://doi.org/10.18203/2349-2902.isj20243238

Sudarman, J. P., Sayudo, I. F., Park, J. Y., Fernandes, A., Machinski, E., Aulia, M. H. U., & Triatmoko, S. E. (2025). The Efficacy of Bromelain-based Enzymatic Debridement in Deep Burn Injury Management: A Comprehensive Systematic Review and Meta-analysis. Journal of Plastic and Reconstructive Surgery. https://doi.org/10.53045/jprs.2024-0051

Tatarusanu, S. M., Lupascu, F. G., Profire, B. S., Szilagyi, A., Gardikiotis, I., Iacob, A. T., Caluian, I., Herciu, L., Gisc?, T. C., Baican, M. C., Crivoi, F., & Profire, L. (2023). Modern Approaches in Wounds Management. Polymers, 15(17). https://doi.org/10.3390/polym15173648

Thedjakusuma, F. W., Nahusuly, F., & Arius, Y. (2022). Perbandingan Tingkat Penyembuhan Luka Bakar Derajat II B ( Deep Dermal ) pada Fase Proliferasi yang Ditinjau dengan Pemberian Larutan Feracrylum 1 %, Tulle , dan Silver Sulfadiazine pada Mencit Mus musculus Abstrak. J. Ked. Mulawarman, 9(3), 122–131.

Thomas, N. A., Taupik, M., Ramadhani, F. N., H. Hutuba, A., & Ramadani Putri Papeo, D. (2024). Penyembuhan Luka Bakar Gel Enzim Bromelin Secara In Vivo. Journal Syifa Sciences and Clinical Research, 6(1), 66–77. https://doi.org/10.37311/jsscr.v6i1.20384

Varilla, C., Marcone, M., Paiva, L., & Baptista, J. (2021). Bromelain, a group of pineapple proteolytic complex enzymes (Ananas comosus) and their possible therapeutic and clinical effects. a summary. Foods, 10(10). https://doi.org/10.3390/foods10102249

Waznah, U., Rahmasari, K. S., Ningrum, W. A., & Slamet. (2021). Bioaktivitas Ekstrak Kulit Buah Nanas (Ananas comosus (L.) Merr.) dalam Sabun Cuci Piring sebagai Antibakteri terhadap Bakteri Staphylococcus aureus. MPI (Media Pharmaceutica Indonesiana), 3(4), 227–234. https://doi.org/10.24123/mpi.v3i4.4721

Wiyono, A. S., Lestari, T. P., Farmasi, F., Ilmu, I., Bhakti, K., & Kediri, W. (2021). Optimasi Formula Gel Ekstrak Kasar Bromelin Kulit Nanas (Ananas Comosus (L.)Merr) Menggunakan Carbopol 940 Dan Gliserin Secara Simplex Lattice Design Optimization Gel Formula Of Bromelin Crude Extract Of Pineapple Peel (Ananas Comosus (L.) Merr) Using Car. Jurnal Pharma Bhakta, 1(2), 55–61. file:///C:/Users/user/Downloads/_8+Anang-2.pdf

Wojciech, Z. (2023). Burns?: Classification , Pathophysiology , and Treatment?: A Review. International Journal Of Molecular Sciences, 14(8), 1–17. https://doi.org/10.7759/cureus.27876

Yulastri, W., Irdawati, I., Yusrizal, Y., Fatiha, F. D., Nissha, D. K., & Anggraeni, N. P. (2024). Innovation in Bromelain Enzyme Production from Pineapple and Honey and Its Characteristics. Jurnal Penelitian Pendidikan IPA, 10(8), 6239–6244. https://doi.org/10.29303/jppipa.v10i8.6976